Yo le otorgaría un 4 a mi proyecto porque hemos hecho mucho avance. Logre terminar de correr los espectros de IR de la muestra de hCen2 WT. Luego, pude hace un análisis de correlación bidimensional (2D-COS analysis). También, tuve la oportunidad de presentar mi proyecto y resultados en el 2D-COS Symposium llevado a cabo el 20-22 de febrero en Rincón of the Seas en Rincón.

He aquí el abstract del trabajo que presenté en el simposio:

FT-IR Spectra and 2D-COS analysis for the thermal perturbation of hCen2 WT

Centrins are calcium binding proteins that are essential for proper chromosome segregation during mitosis in eukaryotic cells. There are 3 human isoforms of Centrin known to date; in this work we will present results for human Centrin2. hCen2 has been associated with having both, structure and regulatory roles within the centrosome. A comparative study of human centrin isoforms: Centrin1 (hCen1), Centrin2 (hCen2), Centrin1 E105K (hCen1 E105K) and Centrin2 E105K (hCen2 E105K) was performed during thermal perturbation by Fourier transform infrared (FT-IR) spectroscopy and analyzed by the method of two-dimensional correlation analysis. 2D-COS spectroscopy allowed us to analyze IR spectra and differentiate overlapping peaks by spreading them over a second dimension. This novel approach is useful to study conformational changes in proteins and specific side-chain interactions. The synchronous and asynchronous contour maps obtained from the centrin samples showed that hCen2 is the most stable when compared to the other Centrins.